Step-by-step Process of PCR test : MED NOW LABS
Step-by-step Process of PCR test
The PCR test includes three basic steps explained below.
Collecting the specimen
Specimen collection is the first and easy step where a health professional uses a swab (a soft piece of material on a stick or rod) to collect respiratory material present in your nose. The swab can be of several different types. It can be a nasal swab that immediately collects a specimen from your nostrils or a nasopharyngeal swab inserted into your nasal cavity to collect the sample. Once the sample is collected, the swab is put and sealed in a tube and moved to the laboratory for further inspection.
Extraction of genetic material
When the sample is received by a healthcare professional at the laboratory, they separate (extracts) the genetic material of the specimen from the other contents of the material.
PCR
This final step of the process involves the amplification of genetic material within the test tube. It is done with the help of several special chemicals, enzymes, and the thermal cycler (a PCR machine). After a number of heating and cooling cycles, the test tube shows millions of copies of the genetic material of the SARS-CoV-2 virus. Now, suppose the SARS-CoV-2 is really present in the genetic material. In that case, it gets detected easily with the help of a chemical that produces a fluorescent light on sensing it in the sample. There are certain indicators that prove the signal is a positive test result sign.
The PCR test is considered highly reliable and accurate for detecting active infection of the SARS-CoV-2 virus. Although most of these tests take hours to be done, some of them are faster too.
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