Elements required for RT-PCR : MED NOW LABS


 Elements required for RT-PCR


RNA Template


A single-stranded RNA extracted from the collected sample
Reverse Transcriptase
It is an RNA-dependent DNA polymerase that uses RNA as a template to catalyze DNA synthesis.
DNA Polymerase
A thermostable polymerase that can work properly at a temperature of 70 and can bear temperatures high as 98 without denaturing.
Primers
Primers are nucleic acid sequences, rather small, that start off the process of DNA synthesis.


Deoxynucleotide triphosphates


These bases provide the energy required for polymerization and give the basic blocks needed for DNA synthesis also.
Buffers


The buffer system is vital for the denaturation and renaturation of DNA. Magnesium and potassium are the most common buffers used to provide favorable conditions for that. Also, these buffers are necessary for the stability, activity, and speed of polymerase.


Thermocycler
It is a laboratory instrument that you can utilize to heat and cool down the samples repetitively in countless cycles. The whole process helps with DNA or RNA amplification through PCR.

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